UK LABORATORIES UNPREPARED
A paper published August 2019 summarises the level of fungal diagnostics in the UK , Amongst the worrying statistics is that whilst 78% of UK laboratories could access local susceptibility testing for yeasts only 17% could access local susceptibility testing for Aspergillus species.
This a copy and paste direct from the EUCAST website;
'Methods of antifungal susceptibility testing
It is recommended that broth micro dilution is used to perform antifungal susceptibility testing of yeasts and moulds.'.
All interpretation is according to EUCAST where EUCAST values are available
Micronaut AM MIC provides laboratories with the possibility to determine the MIC of up to 9 antimycotics on a single plate.
For details of antimycotics included download attached file. Alternatively with the top 6 agents only, two samples can be processed on a single plate product code M/E1-832-040
All 9 agents are available on a single plate. for a single sample in product code M/E1-831-040
The method used is broth microdilution described in ISO 20776-1:2006 referred to in EUCAST documents.
Features and benefits
• Standardized MIC determination of antifungal agents with the newly composed MICRONAUT 1640 medium + MOPS + Glucose for application in routine laboratory diagnostics.
• Due to the spectrum of antifungal agents, the MIC plate is appropriate for testing yeasts and cryptococci originating from hospital and community acquired infections
• Growth of the fungi is indicated by a color change from blue to pink mediated by the AFST indicator supplemented to the test medium.
• Easy visual or photometric reading and evaluation of the AFST results is provided by the colour change of the AST indicator
• The MICRONAUT software provides reading, evaluation and interpretation according to the latest EUCAST criteria.
Shelf life and storage
• Shelf life: 24 months from date of production
• Storage: at room temperature (15-25°C)
BioConnections has been helping UK laboratories implement manual broth microdilution for several years.
• Prepare a 0.5 McFarland bacteria suspension in NaCl
• Transfer an aliquot into MICRONAUT-RPMI 1640 medium + MOPS + Glucose
• Add AST indicator (Redox dye)
• Inoculate the MICRONAUT-AM Antifungal Agents MIC plate
• Incubate for 24 hours for yeast and 48 hours for Cryptococci at 35-37°C
• Read the result visually or measure photometrically