Direct Detection
Although RESIST was initially developed for use on conventional overnight culture Coris BioConcept and many scientists recognise its potential for detecting and identifying carbapenemases without the need for overnight culture..
Below is a collection of references to work done exploring RESIST's potential.
Blood cultures - A positive blood culture can be treated with the standard protocol following lysis and concentration steps. Carbapenemase detection and identification is obtained within one hour of a blood culture flagging positive.
Rectal swabs - Following a period of enrichment in broth supplemented with a carbapenem sensitive and specific results are obtained. This has the potential to reduce carbapenemase screening to a matter of hours at a significantly lower cost than PCR options.
Development of validated protocols is underway
Below is a collection of references to work done exploring RESIST's potential.
Blood cultures - A positive blood culture can be treated with the standard protocol following lysis and concentration steps. Carbapenemase detection and identification is obtained within one hour of a blood culture flagging positive.
Rectal swabs - Following a period of enrichment in broth supplemented with a carbapenem sensitive and specific results are obtained. This has the potential to reduce carbapenemase screening to a matter of hours at a significantly lower cost than PCR options.
Development of validated protocols is underway