RESIST direct

Direct detection - Publications Blood cultures Rectal Other

P0293 – Reduction in the detection time of OXA-48, OXA-163 and KPC carbapenemases performed with a novel immunochromatographic lateral flow assay from 2-hour-old cultures (early cultures).
F. Pasteran, L. Denorme, Q. Gilleman, S. Gomez, E. Albornoz, Y. Glupczynski, P. Bogaerts, P. Mertens and A. Corso.
27th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 22 – 25, 2017
Poster

p0293_abstract__1_.pdf
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0128 – Carbapenem resistant Enterobacteriaceae (CRE) surveillance can be performed using faecal samples obtained for C.difficile toxin detection without the need for a rectal swab.
M. Wilks, A. Whiley, A. Alcolea-Medina and B.P. Cherian.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018

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• Lateral flow immunochromatographic assay for rapid screening of faecal carriage of carbapenemase-producing Enterobacteriaceae.
Fauconnier C, Dodemont M, Depouhon A, Anantharajah A, Verroken A, Rodriguez-Villalobos H.
J Antimicrob Chemother. 2018 Oct 30
Abstract
Background:
Rapid and effective screening of carbapenemase-producing Enterobacteriaceae (CPE) appears essential for adequate patient management and rapid implementation of infection control measures. Most of these screening techniques require a minimum of 24 h of culture. Molecular assays are an exception since these can be achieved within 1 h, but are expensive and usually require specialized facilities and trained personnel. In this context, lateral immunochromatography performed directly from rectal swab samples could represent a cost-effective alternative with a reduced turnaround time.
Objectives: In this study, we assessed the performance of the OKN K-SeT test (Coris BioConcept, Gembloux, Belgium) for the rapid detection of OXA-48, KPC and NDM CPE directly from rectal swab samples.
Methods: A total of 149 residual rectal swabs, routinely screened for CPE through selective culture and confirmed by PCR, were tested with a defined protocol consisting of a 2.5 h incubation of the swab in an enrichment medium containing meropenem followed by OKN K-SeT testing after centrifugation.
Results:This method displayed an overall sensitivity of 96% and a specificity of 100% with a limit of detection ranging between 104 and 105 cfu/mL.
Conclusions: Whereas this assay appears highly specific, it displays a reduced sensitivity compared with the standard procedure encompassing a culture step. Nonetheless, this rapid method allows an accelerated identification of most CPE carriers at a lower cost and, accordingly, the implementation of early appropriate management procedures.
https://www.ncbi.nlm.nih.gov/pubmed/30376099

• P1064 – Rectal screening of OXA-48-producing Enterobacteriaceae.
S.A. Gibaud, E.Thomas, L. Crémet and J. Caillon.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018
Poster

• P2332 – Carbapenemase-producing Enterobacteriaceae (CPE) detection directly from surveillance rectal swab by immunochromatographic test.
C.R.L. Fonseca, A.V. Braga, C.R. Ferrari, T. Chagas-Neto, A.C. Ramos, A. C.Gales, A. Andriolo and C.G. Carvalhaes.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018

p2332_-_fonseca_and_carvalhaes_eccmid2018_poster.pdf
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Detection of OXA-370 directly from rectal swabs and blood culture vials using an immunochromatographic assay.
Nodari CS, Gales AC, Barth AL, Magagnin CM, Zavascki AP, Carvalhaes CG.
J Microbiol Methods. 2017 May 5;139:92-94. doi: 10.1016/j.mimet.2017.05.003.
Abstract
We evaluated the performance of OXA-48 K-SeT assay for detecting OXA-370 directly from spiked rectal swabs and blood culture vials. The limit of detection of this test was 104UFC/mL for rectal swabs. Detection of the OXA-370-producing isolates was successfully achieved directly from positive blood culture vials independent of growing conditions.
http://www.sciencedirect.com/science/article/pii/S0167701217301136

• 00810 – Rapid detection of carbapenemase-producing Enterobacteriaceae directly from positive blood cultures by a new immunochromatographic assay.
A.Hamprecht, H. Seifert and A. Saleh.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster

Detection of carbapenemases-producing Enterobacteriaceae in positive blood culture using Immunochromatographic RESIST-4 O.K.N.V assay.
Cointe A, Bonacorsi S, Truong J, Hobson C, Doit C, Monjault A, Bidet P, Birgy A.
Antimicrob Agents Chemother. 2018 Sep 24. pii: AAC.01828-18. doi: 10.1128/AAC.01828-18.
Abstract
We evaluated the performances of the RESIST-4.O.K.N.V assay (Coris®) on 98 isolates to detect OXA-48-like, KPC-, NDM- and VIM-type carbapenemases directly on positive human blood cultures. OXA-48-like and KPC-type isolates were correctly detected but detection of NDM and VIM-type was weak and variable. We show that repeating the test on a 4-hour subculture improves the detection of NDM- and VIM-type to 100%.
https://www.ncbi.nlm.nih.gov/pubmed/30249695

Evaluation of the KPC K-SeT® immunochromatographic assay for the rapid detection of KPC carbapenemase producers from positive blood cultures.
Riccobono E, Antonelli A, Pecile P, Bogaerts P, D'Andrea MM, Rossolini GM.
J Antimicrob Chemothes.
https://www.ncbi.nlm.nih.gov/pubmed/29126269
2018 Feb 1;73(2):539-540. doi: 10.1093/jac/dkx406.
In this work, we evaluated the performance of the KPC K-SeT® assay for the detection of KPC-type carbapenemases directly from positive blood culturer

• P2333 - A new method for detection of carbapenemase-producing Enterobacteriaceae carriage.
K.Villageois-Tran, A.A. Mariaggi, A. Godmer, M. Lambot, T. Leclitpeux, G. Arlet, Y. Benzerara and S. Gallah.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster

Détection directe des carbapénèmases sur hémoculture par test immunochromatographique RESIST-4-O.K.N.V.
Cointe A, Bonacorsi S, Truong J, Hobson C, Doit C, Monjault A, Bidet P, Birgy A.
38e Réunion interdisciplinaire de chimiothérapie anti-infectieuse 17-18 décembre, 2018 Paris.
Poster

• P2325 – Rapid detection of carbapenemase-producing organisms directly from blood cultures using a combined MALDI-TOF MS and RESIST O.K.N. workflow.
E. Wey, L. Ainsworth, T. McHugh and I. Balakrishnan.
28th European Congress of Clinical Microbiology and Infectious Diseases, Infectious Diseases April 21 – 24, 2018.
Poster

Direct Detection