Real-time PCR Internal Controls
Optimal positive control for DNA/RNA extraction and PCR conditions
> Perfect internal control
> Taqman style probe
> Available to read through CY5, FAM, FAMRA, VIC or ROX channel
> Primer limited to prevent interference with target gene primers
Negative test results are only valid when the suitability of the reagents, template and protocols have also been demonstrated. The Primerdesign real-time PCR internal control kit contains either control RNA or DNA. This can be spiked in to your sample prior to nucleic acid extraction or diluted and spiked directly in to your real-time PCR reaction. A positive signal of around Cq 25 indicates that your experimental procedure is working well. The internal control kit can be multiplexed, or used in parallel with your test samples. The assay is primer limited and based on an obscure nucleic acid sequence so will not therefore interfere with the detection of your target sequence.
Kit contents: Lyophilised positive control primers/probe (150 reactions), positive control DNA or RNA template, RNAse/DNAse free water. cost; £125
Precision Onestep qRT-PCR Mastermix
> Huge cost saving
> As good or better than leading brands
> Precise reproducible results
> Available premixed with SYBR green at no extra charge
Our Onestep qRT-PCR MasterMix is a convenient, one step, closed tube reaction which means reduced setup/handling time as well as reduced risk of contamination. Our optimised rapid protocol includes a 10 minute RT step. It is also ideal for RNA pathogen detection. Just add primers and template for a perfect real-time PCR reaction.
Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 quality management laboratory environment.
Kit contains: 4.2ml (400 reactions) Precision OneStep qRT-PCR MasterMix cost; £185