Coronovirus Strain 2019-nCoVMONODOSE dtec-qPCR Test
As of 19-Jan-2020, 13 genome sequences from 6 different labs were released onto GISAID. Our colleagues at Genetic PCR Solutions analysed the set of data and designed primers and probes fully specific for this new corona virus. The real time PCR (qPCR) was adapted to their innovative MONODOSE format, which consists of single PCR reactions tubes containing all reagents (dehydrated), to which only the extracted sample needs to be added. The product is compatible with all open qPCR systems.
Cost per test £4.87 to £5.37 depending on pack size.
About the MONODOSE dtec-qPCR Test format
“New format, dehydrated and ready-to-use”
This innovative product provides individual, ready-to-use, tubes containing all the components needed for detection of specific pathogens: dNTPs, polymerase, pathogen-specific primers and probe, and the appropriate buffer.
Dispensing aliquots for PCR from a master solution, with addition of primer/probe, has some disadvantages: rupture of the enzyme by freezing/thawing, risks of cross-contamination, fluorophore deterioration by UV light and time consuming. Some benefits are listed below:
Just add your diluted sample to each tube and run qPCR.
As reagents are dehydrated these kits can be transported at room temperature.
Optimised MONODOSE dtec-qPCR Tests shows no difference compared to the non-dehydrated ordinary qPCR.
Our HotStart Taq polymerase is very stable in absence of water and is rapidly activated with only 1 minute at 95ºC.
Thermal regime is rapid and the same for all our kits, enabling detection of different pathogens in the same run.
Compatibility with all qPCR devices, plate based and glass capillary.
Take the MONODOSE tubes you need to test from the fridge, no need defrosting, no risk of contamination... ...Just add sample and run PCR with our standard protocol.